PCR Papers

Genetic Engineering with PCR Robert M. Horton and Robert C. Tait from Genetic Engineering with PCR

Endonuclease-Mediated Long PCR and Its Application to Restriction Mapping
Curr. Issues Mol. Biol. (1999) 1 (2): 77-88 Chengtao Her and Richard M. Weinshilboum
The polymerase chain reaction (PCR) is the most widely used technique for the study of DNA. Applications for PCR have been extended significantly by the development of "long" PCR.

A PCR-based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence
Curr. Issues Mol. Biol. (1999) 1 (1): 47-52 Catherine A. Boulter and Dipa Natarajan
A simple PCR-based method for the isolation of genomic DNA that lies adjacent to a known DNA sequence.

Universal TA Cloning
Curr. Issues Mol. Biol. (2000) 2 (1): 1-7 Ming-Yi Zhou and Celso E. Gomez-Sanchez
TA cloning is one of the simplest and most efficient methods for the cloning of PCR products.

Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
Curr. Issues Mol. Biol. (2002) 4: 13-18 Clifford F. Brunk, Jinliang Li and Erik Avaniss-Aghajani
The use of quantitative PCR for measuring bacterial abundance in environmental samples.

PCR Clamping
Curr. Issues Mol. Biol. (2000) 2 (1): 27-30 Henrik Ørum
An efficient, PCR based method for the selective amplification of DNA target sequences that differ by a single base pair. The method utilises the high affinity and specificity of PNA for their complementary nucleic acids and that PNA cannot function as primers for DNA polymerases.

DNA Splicing by Directed Ligation (SDL)
Curr. Issues Mol. Biol. (1999) 1 (1): 21-30 DNA Yuri A. Berlin
Splicing by directed ligation (SDL) is a method of in-phase joining of PCR-generated DNA fragments that is based on a pre-designed combination of class IIS restriction endonuclease recognition and cleavage sites.